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Instructor/Advisor

Anastassiia Vertii

Keywords

Giemsa staining, Mitotic cell cycle stages, hTERT RPE-1 cells, HeLa cells

Abstract

This study investigated the differences in cell division stages between normal and cancer human cells. Specifically, normal diploid retinal epithelial cells, hTERT-immortalized female retinal pigment epithelial cell line, hTERT RPE-1, were derived by transfecting the RPE-340 cell line with the pGRN145 hTERT-expressing plasmid (ATCC MBA-141) and served as control. Additionally, HeLa cells, female epithelial cells isolated from adenocarcinoma tumor (ATCC-CCL2), the first immortal human cells to be grown in culture, were analyzed as an example of cancer cells. The results suggest remarkable differences in mitotic index and defects during mitosis.

Giemsa staining revealed differences in cell cycle between normal diploid and cancer cells

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