Date of Award

Spring 2019

First Advisor

Mark Birnbaum


Through this semester-long experiment, professor Birnbaum and I attempted to identify the presence of BRPF1 (Bromodomain And PHD Finger Containing 1) in harvested RAW osteoclast cells. This was done through a series of steps, beginning with growing RAW osteoclasts, differentiating them, harvesting them and finally running a Western Blot test to look for our target protein. Unfortunately, no major discoveries were made with this project, but there is still reason to be optimistic about the presence of other members of the BRPF family (BRPF3, BRPF4). With some knowledge on our procedure, future researchers should be able to note specific points that could be tweaked for improvement to yield better results.

Included in

Biology Commons